Part:BBa_K802009:Design

Sufactin generator and biofilm repressor for B. subtilis

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 760
  Illegal SapI.rc site found at 770
  

Design Notes

The part has 3 genes (sfp, abrB and lacI) under the control of the xylose inducible P_xyl promoter.

Source

The part was constructed from the assembly of 3 biobricks: BBa_K802006,BBa_K802007 and BBa_K802008.

References

1. Fernandes, P.A.V. et al., Antimicrobial activity of surfactants produced by Bacillus subtilis R14 against multidrug-resistant bacteria, Braz. J. Microbiol. vol.38 no.4 São Paulo Oct./Dec. 2007;
2. Al-Ajlani, M.M. et al., Production of surfactin from Bacillus subtilis MZ-7 grown on pharmamedia commercial medium (2007);
3. Abushadi, H.M. et al., Molecular Characterization of Bacillus subtilis Surfactin Producing Strain and the Factors Affecting its Production, Int. J. Agri. Biol., Vol. 7, No. 3, 2005;
4. Techaoei,S. et al., Preliminary screening of biosurfactant producing microorganisms isolated from hot spring and garages in Northern Thailand,KMITL Sci. Tech. J. Vol. 7 No. S1 Nov. 2007